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Research Update

Optimizing Gene Editing with a Combination of mRNA and AAV

In the past few years there have been major advances towards fast, efficient, and precise gene editing. However, there are still several hurdles to overcome, particularly for certain cell types. Hematopoietic stem and progenitor cells (HSPCs) are notoriously hard to edit, especially in cases where the donor DNA template is large, such as when the goal is to replace an entire gene. And even more challenging, is to target the most primitive CD34+CD133+CD90+ HPSC cell population, which contains the repopulating hematopoietic stem cells necessary for long-term engraftment and differentiation.

In a recent paper published in Nature Biotechnology, researchers led by Michael Holmes and Paula Cannon sought to make this process more efficient through the use of...

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The manufacturing of therapeutic mRNA has several challenges. Standard purification processes leave behind small amounts of biological contaminants and impurities. While this might be acceptable for some research applications, therapeutic mRNA must have the highest purity possible. Previously, methods to test for and reduce variation have been insufficient.

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Ask an Expert

What base modifications can be incorporated into my custom mRNA or long RNA?

TriLink offers a variety of modified rNTPs suitable for in vitro transcription. We have assessed transcription efficiency based on final product formation of a 1.9 kb transcript with the following triphosphates...

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Zone In With Zon Blog Post

Big, Bigger, Biggest?Genomics Projects Go Democratic

This blog on genomics projects going democratic has genomics going from singular to pluralistic. Let me frame this revolutionary change another way...

Zone In on December 15th for my new in depth blog post "Longing for Longevity."

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